Comparative Nutritional Profiling of Leucaena leucocephala (Wondergraze and Taramba varieties) for Enhanced Animal Feed Applications
DOI:
https://doi.org/10.51601/ijse.v6i1.422Abstract
Leucaena leucocephala belongs to the family Leguminosae and is one of the fastest-growing leguminous trees. L. leucocephala leaves have a great potential as an alternative protein source for animal feed. However, the presence of anti-nutritional compounds, such as mimosine and tannins, limits their direct utilisation by animals, as these compounds can reduce nutrient digestibility and impair animal performance. Ensiling has been widely recognised as an effective processing method to reduce these compounds. This study evaluated the proximate composition, fibre fractions, mineral composition, and anti-nutritional compounds of unensiled (fresh) and ensiled L. leucocephala leaves from Wondergraze and Taramba varieties to assess their potential as animal feed resources. Overall, the ensiling process significantly influenced the nutritional profiles of L. leucocephala leaves in both Wondergraze and Tarramba varieties. Ensiling enhanced crude protein content while reducing crude fibre, NDF and moisture levels, indicating improved digestibility and feed intake potential. Although slight reductions in ash content, gross energy and certain minerals were observed, most nutrients were largely retained, suggesting that fermentation did not compromise the overall nutritional value of the forage. Varietal differences were evident, with Wondergraze demonstrating better fibre preservation and smaller energy losses compared to Tarramba, highlighting its potentially superior fermentation efficiency. Importantly, ensiling markedly reduced anti-nutritional compounds, particularly mimosine and tannins, thereby improving feed safety and suitability. These findings demonstrate that ensiling is an effective processing method to enhance the nutritional quality and practical utilisation of L. leucocephala leaves as animal feeds, while also revealing cultivar-specific responses that are valuable for targeted feed formulation and forage management strategies.
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[1] Angelis, A. D., Gasco, L., Parisi, G., & Danieli, P. P. (2021). A Multipurpose Leguminous Plant for the Mediterranean Countries: Leucaena leucocephala as an Alternative Protein Source: A Review. Animals (Basel), 11(8), 2230. 10.3390/ani11082230
[2] AOAC International. (2023). Official methods of analysis (22nd ed.). AOAC International.
[3] Charmley, E., McSweeney, C. S., Bishop-Hurley, G. J., Simington, J., Padmanabha, J., & Giacomantonio, P. (2023). Evaluation of the productivity and feed value of Wondergraze and Redlands leucaena cultivars under grazing. Animal Production Science, 63(3), 450–462. https://doi.org/10.1071/AN22260
[4] Chanchay, N., & Poosaran, N. (2009). The reduction of mimosine and tannin contents in leaves of Leucaena leucocephala. Asian Journal of food and agro-industry, 2, 137-144.
[5] Desta, S.T., Yuan, X., Li, J., & Shao, T. (2016) Ensiling characteristics, structural and nonstructural carbohydrate composition and enzymatic digestibility of Napier grass ensiled with additives. Bioresource Technology, 221, 447-454. doi: 10.1016/j.biortech.2016.09.068. Epub 2016 Sep 17. PMID: 27668877.
[6] Fayemi, P. O., Onwuka, C. F. I., Isah, O. A., Jegede, A. V., Arigbede, O. M., & Muchenje, V. (2011). Effects of mimosine and tannin toxicity on rabbits fed processed Leucaena leucocephala (Lam) De Wit leaves. African Journal of Agricultural Research, 6(17), 4081–4085. 10.5897/AJAR11.327
[7] Food and Agriculture Organization of the United Nations. (n.d.). Feed storage practices: moisture and safe storage of animal feed. FAO. Retrieved: 2 January 2026, from https://www.fao.org/4/s4314e/s4314e08.htm
[8] Hardiansyah, B., Tafsin, M., Daulay, A. H., Wahyuni, T. H., & Ginting, N. (2017). Effect of processing Lamtoro leaf (Leucaena leucocephala) as feed on rex rabbit carcass. Jurnal Peternakan Integratif, 5(3). https://doi.org/10.21776/ub.jpi.2017.005.03
[9] Honda, M. D. H., & Borthakur, D. (2019). Mimosine concentration in Leucaena leucocephala under various environmental conditions. Tropical Grasslands–Forrajes Tropicales, 7(2), 164–172. https://doi.org/10.17138/tgft(7)164-172
[10] Jiang, X., Liu, X., Xu, H., Sun, Y., Zhang, Y., & Wang, Y. (2021). Improvement of the nutritional, antioxidant and bioavailability properties of corn gluten-wheat bran mixture fermented with lactic acid bacteria and acid protease. LWT, 144, 111161. https://doi.org/10.1016/j.lwt.2021.111161
[11] Khamseekhiew B, Jansuk J, Puddang S, Nakavirot P, & Pimpa O. (2022). Effect of turmeric (Curcuma longa Linn.) powder supplements in diet on milk yield of lactating goats. Khon Kaen Agriculture Journal (Supplement), 1, 284-291.
[12] Knez, E., Kadac-Czapska, K., & Grembecka, M. (2023). Effect of Fermentation on the Nutritional Quality of the Selected Vegetables and Legumes and Their Health Effects. Life (Basel), 13(3), 655. 10.3390/life13030655
[13] Kung, L. R. D., Shaver, R. J., Grant, R. J., & Schmidt (2018). Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science, 101 (5), 4020-4033. https://doi.org/10.3168/jds.2017-13507
[14] Li, Y., Niu, L., Guo, Q., Shi, L., Deng, X., Liu, X., & Xiao, C. (2022). Effects of fermentation with lactic bacteria on the structural characteristics and physicochemical and functional properties of soluble dietary fiber from prosomillet bran. LWT, 154, 112609. https://doi.org/10.1016/j.lwt.2021.112609
[15] Mokoboki, H. K., Sebola, A. N., Ravhuhali, K. E., & Nhlane, L. (2019). Chemical composition, in vitro ruminal dry matter degradability, and dry matter intake of some selected browse plants. Journal of Food and Agriculture, 5, 1587811. https://doi.org/10.3389/fnut.2019.1587811
[16] Montoya-Flores. M. D.. Botero. I. C.. Arango. J.. Romano-Munoz. J. L.. Solorio-Sanchez. F. J.. and Aguilar-Perez. C. F.. and Ku-Vera. J. C. (2020). Effect of dried leaves of Leucaena leucocephala on rumen fermentation. rumen microbial population. and enteric methane production in crossbred heifers. Animals. 10(2). 300. Rabbits. and Rodents: Clinical Medicine and Surgery. 4th Edition. 174–187. doi: 10.1016/B978-0-323-48435-0.00014-9.
[17] Nulik, J., Dahlanuddin, Hau, D. K., Pakereng, C., Edison, R. G., Liubana, D., Ara, S. P., & Giles, H. E. (2013). Establishment of Leucaena leucocephala cv. Tarramba in eastern Indonesia. Proceedings of the 22nd International Grassland Congress.
[18] Oyun, M. B. (2006). Chemical characterization of selected tree legumes as indices for their litter quality. Journal of Applied Sciences, 6(10), 2321–2324. https://doi.org/10.3923/jas.2006.2321.2324
[19] Raju, S., Nagalakshmi, D., Kumari, N. N., & Sravanthi, B. (2025). Nutritional Characterisation of Leucaena Leucocephala Leaves: Insights for Sustainable Ruminant Production. Journal of Experimental Agriculture International, 47(6), 274-80. https://doi.org/10.9734/jeai/2025/v47i63487.
[20] Sakhawat, I. (2011). The effect of silage quality on gross energy losses. Swedish University of Agricultural Sciences.
[21] Shahidi, F., & Oh, W. Y. (2020). Lipid-derived flavor and off-flavor of traditional and functional foods: an overview. Journal of Food Bioactives, 10.
[22] Thamaga, M., & Mokoboki, T. (2021). Apparent digestibility and nutritional composition of Leucaena. Tropical Animal Health and Production, 53, 458. https://doi.org/10.1007/s11250-021-02668-2
[23] Wan-Mohd-Nazri, W. A. R., Johari, N. A. N., Sarmin, S. N., Yunus, N. Y. M., Japarudin, Y., Mahmud, J., & Khairuddin, M. N. (2020). Leucaena leucocephala: A fast-growing tree for the Malaysian particleboard industry. BioResources, 15(4), 7433–7442. https://doi.org/10.15376/biores.15.4.7433-7442
[24] Wang, S. (2025). Silage preparation, processing and efficient utilization. Agriculture, 15(2), 128. https://doi.org/10.3390/agriculture15020128
[25] Wang, Y., Wang, H., Wu, Y., Xiang, H., Zhao, Y., Chen, S., Qi, B., & Li, L. (2022). Insights into lipid oxidation and free fatty acid profiles to the development of volatile organic compounds in traditional fermented golden pomfret based on multivariate analysis. LWT, 171, 114112. https://doi.org/10.1016/j.lwt.2022.114112
[26] Zhao, G., Wu, H., Li, Y., Huang, Z., He, J., & Xie, X. (2025). Ensiling characteristics, in vitro digestibility and bacterial community structure of mulberry leaf silage with or without the addition of cellulase, protease, and starch. Frontiers in Plant Science, 16, 1517529.
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