First report of powdery mildew caused by Podosphaera xanthii on Ageratum conyzoides, in Indonesia
DOI:
https://doi.org/10.51601/ijse.v6i1.415Abstract
Powdery mildew is one of the most common and important fungal diseases and may cause huge economic losses to crop yields worldwide. During survey of powdery mildews in 2011 and 2013, six samples of Ageratum conyzoides were collected from different regions in Indonesia, i.e. Bali, South Sumatera and West Java provinces. Anamorphic features revealed that the fungus belongs to the genus Podosphaera. Two sets of sequences of both ITS rRNA and 28S regions were obtained from the six samples. Phylogenetic analyses, including maximum parsimony (MP) and maximum likelihood (ML) were executed using MEGA7. The strength of internal branches of the resulting trees were tested with bootstrap analysis. Tree scores, including tree length, CI, RI and RC were also calculated. The phylogenetic analysis confirmed that the fungus belongs to the genus Podosphaera, forming a clade with the sequences from Podosphaera xanthii. This is the first report of powdery mildews on Ageratum conyzoides from Indonesia.
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[1]. Afshan, N. S., Jabeen, M., Akbar, A., Khalid, M., Saleem, A., Altaf, R., Afzal, S., Fiaz, M., Khalid, A. N. (2025). Geographic distribution and genetic diversity of Podosphaera xanthii in Pakistan. Asian Journal of Plant Pathology 19(1):81–95.
[2]. https://doi.org/10.3923/ajpp.2025.81.95
[3]. Amano (Hirata), K. (1986). Host range and geographical distribution of the powdery mildew fungi. Japan Scientific Societies Press, Tokyo
[4]. Arnold, A. E., Maynard, Z., Gilbert, G. S., Coley, P.D., Kursar, T. A. (2000). Are tropical fungal endophytes hyperdiverse. Ecology Letter 3: 267–274.
[5]. https://doi.org/10.31046/j.1461-0248.2000.00159.x
[6]. Aryuti, T., Rifai, M. A. (1987). Marga-marga jamur embun tepung di Indonesia. Floribunda 1(3): 9–12.
[7]. Bademiyya, S. I., Ashtaputre, S. A. (2019). Estimation of yield loss due to powdery mildew of chilli caused by Leveillula taurica (Lev.) Arn. International Journal of Pure & Applied Bioscience 7(1): 323–326.
[8]. http://dx.doi.org/10.18782/2320-7051.7347
[9]. Bradshaw, M., Boufford, D., Braun, U., Moparthi, S., Jellings, K., Maust, A., Pandey, B., Slack, S., Pfister, D. (2024). An in depth evaluation of powdery mildew hosts reveals one of the world’s most common and widespread groups of fungal plant pathogens. Plant Disease 108:576–581.
[10]. https://doi.org/10.1094/PDIS-07-23-1471-RE
[11]. Braun, U. (1987). A monograph of the Erysiphales (powdery mildews). Beihefte zur Nova Hedwigia 89: 1–700.
[12]. Braun, U. (2011). The current systematic and taxonomy of powdery mildews (Erysiphales): an overview. Mycoscience 52:210–212.
[13]. https://doi.org/10.1007/S10267-010-0092-1
[14]. Braun, U., Cook, R. T. A. (2012). Taxonomic manual of the Erysiphales (powdery mildews). CBS Biodiversity series No. 11. CBS-KNAW Fungal Biodiversity Centre, the Netherlands.
[15]. Edgar, R. C. (2004). MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792‒1797. https://doi.org/10.1093/nar/gkh340
[16]. Felsenstein, J. (1985). Confidence limits on phylogenetics: an approach using the bootstrap. Evolution 39: 783‒791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
[17]. Gent, D. H., Grove, G. G., Wolfenbarger, S. N., Woods, J. L. (2014). Crop damage caused by powdery mildew on hop and its relationship to late season management. Plant Pathology 63: 625–639.
[18]. https://doi.org/10.1111/ppa.12123
[19]. Glawe, D. A. (2008). The powdery mildews: A review of the world’s most familiar (yet poorly known) plant pathogens. Annual Review of Phytopathology 46: 27–51.
[20]. https://doi.org/10.1146/annurev.phyto.46.081407.104740
[21]. Hirata, T., Takamatsu, S. (1996). Nucleotide sequence diversity of rDNA internal transcribed spacer extracted from conidia and cleistothecia of several powdery mildew fungi. Mycoscience 37:265–270.
[22]. https://doi.org/10.1007/BF02461299
[23]. Kelly, L. A., Vaghefi, N., Bransgrove, K., Fechner, N. A., Stuart, K., Pandey, A. K., Sharma, M., Nemeth, M. A., Liu, S., Tang, S.R., Nair, R. M., Douglas, C. A., Kiss, L. (2021). One crop disease, how many pathogens? Podosphaera xanthii and Erysiphe vignae sp. nov. identified as the two species that cause powdery mildew of mungbean (Vigna radiata) and Black gram (V. mungo) in Australia. Phytopathology 111(7): 1193–1206. https://doi.org/10.1094/PHYTO-12-20-0554-R
[24]. Kishino, H., Hasegawa, M. (1989). Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in Hominoidea. Journal of Molecular Evolution 29: 170–179. https://doi.org/10.1007/BF02100115
[25]. Kumar, S., Stecher, G., Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0. for bigger datasets. Molecular Biology and Evolution 33: 1870–4.
[26]. Limkaisang, S., Cunnington, J. H., Liew, K. W., Salleh, B., Sato, Y., Divarangkoon, R., Fangfuk, W., To-anun, C., Takamatsu, S. (2006). Molecular phylogenetic analyses reveal close relationship of powdery mildew fungi on some tropical trees with Erysiphe alphitoides, an oak powdery mildew. Mycoscience 47: 327–335. https://doi.org/10.1093/molbev/msw054
[27]. Meeboon, J., Hidayat, I., Kramadibrata, K., Nurcahyanto, D., Siahaan, S. A. S., Takamatsu, S. (2012a). Cystotheca tjibodensis (Erysiphaceae, Ascomycota): rediscovery in Java after 90 years and first finding of anamorph. Mycoscience 53: 386–390. https://doi.org/10.1007/S10267-011-0176-6
[28]. Meeboon, J., Hidayat, I., Takamatsu, S. (2012b). Erysiphe javanica sp. nov., a new tropical powdery mildew from Indonesia. Mycotaxon 120: 189–194. https://dx.doi.org/10.5248/120.189
[29]. Meeboon, J., Hidayat, I., Takamatsu, S. (2013a). Pseudoidium javanicum, a new species of powdery mildew on Acalypha spp. from Indonesia. Mycoscience 54: 183–187. https://doi.org/10.1016/j.myc.2012.08.006
[30]. Meeboon, J., Hidayat, I., Takamatsu, S. (2013b). Setoidium castanopsidis, a new species of anamorphic Cystotheca (Ascomycota, Erysiphales) from Indonesia. Mycoscience 54: 274–278.
[31]. https://doi.org/10.1016/j.myc.2012.10.004
[32]. Meeboon, J., Kokaew, J., Takamatsu, S. (2018). Notes on powdery mildews (Erysiphales) in Thailand V. Golovinomyces. Tropical Plant Pathology 43(3): 202–217. https://doi.org/10.1007/s40858-017-0201-1
[33]. Meena, N. K., Trivedi, A., Kumar, S., Jatwa, T. K., Meena, S. C. (2022). Survey and surveillance to observe occurrence and severity of powdery mildew of mungbean (Vigna radiata L.) growing areas around Udaipur region. The Pharma Innovation Journal 11(2): 960–963.
[34]. Mukhtar, I., Arend, F. P. (2017). First report of Podosphaera xanthii causing powdery mildew on Ageratum conyzoides in China. Plant Disease 101(8), p.1553. https://doi.org/10.1094/PDIS-03-17-0448-PDN
[35]. Palm, B. T. (1921). Een gevaar voor de tabakscultuur in Deli. Bull Deli¬ Proefstat te Medan–Sumatra, Indonesia RAM 1:275
[36]. Raciborski, M. (1900). Parasitische Algen und Pilze Javas. Teil I. Batavia (Djakarta). Bot Inst zu Buitenzorg, Indonesia
[37]. Reddy, D. B. (1970). List of diseases of important economic crop plants of Indonesia. Techn Docum., FAO Plant Prot Comm S. E. Asia and Pacific Region No. 74
[38]. Savary, S., Willocquet, L., Pethybridge, S. J., Esker, P., McRoberts, N., Nelson, A. (2019). The global burden of pathogens and pests on major food crops. Nature Ecology and Evolution (3)430–439.
[39]. https://doi.org/10.1038/s41559-018-0793-y
[40]. Schwarz, M. B. (1926). Meeldauw van tabak en Physalis minima. Indische Culturen (Teysmannia) 11:238–239 RAM 5: 634
[41]. Schwarz, M. B. (1927). Oidium verbenae nov. sp.: Meeldauw van Verbena laciniata. Indische Culturen (Teysmannia), 12: 470–471 RAM 7:32
[42]. Schweizer, J. (1928). Over Erysiphaceen (meeldauwschimmels) van Java. Arch v Rubbercult Nederl Indie 12: 323–343
[43]. Semangun, H. (1992). Host index of plant diseases in Indonesia. Gadjah Mada University Press, Indonesia.
[44]. Shimodaira, H., Hasegawa, M. (1999). Multiple comparison of log-likelihoods with the applications to phylogenetic inference. Molecular Biology and Evolution 16: 1114–1116. https://doi.org/10.1093/oxfordjournals.molbev.a026201
[45]. Shin, H. D, La, Y. J. (1993). Morphology of edge lines of chained immature conidia on conidiophores in powdery mildew fungi and their taxonomic significance. Mycotaxon 66: 445–451.
[46]. Shirouzu, T., Takamatsu, S., Hashimoto, A., Meeboon, J., Ohkuma, M. (2020). Phylogenetic overview of Erysiphaceae based on nrDNA and MCM7 sequences. Mycoscience 61:249–258. https://doi.org/10.1016/j.myc.2020.03.006
[47]. Siahaan, S. A. S., Hidayat, I., Kramadibrata, K., Meeboon, J., Takamatsu, S. (2015). Phyllactinia poinsettiae sp. nov.: a new species of powdery mildew on poinsettia from Indonesia. Mycoscience 56: 580–583. https://doi.org/10.1016/j.myc.2015.05.005
[48]. Siahaan, S. A. S., Hidayat, I., Kramadibrata, K., Meeboon, J., Takamatsu, S. (2016a). Erysiphe baliensis and E. sidae, two new species of anamorphic Erysiphe (powdery mildew) from Indonesia. Mycoscience 57: 35–41. https://doi.org/10.1016/j.myc.2015.08.001
[49]. Siahaan, S. A. S., Hidayat, I., Kramadibrata, K., Meeboon, J., Takamatsu, S. (2016b). Bauhinia purpurea, Durio zibethinus and Nephelium lappaceum: additional hosts of the asexual morph of Erysiphe quercicola. Mycoscience 57: 375–383. https://doi.org/10.1016/j.myc.2016.06.001
[50]. Siahaan, S. A. S., Hidayat, I., Kramadibrata, K., Meeboon, J., Takamatsu, S. (2016c). Podosphaera perseae-americanae, a new powdery mildew species on Persea americana (avocado) from Indonesia. Mycoscience 57: 417–421. https://doi.org/10.1016/j.myc.2016.07.004
[51]. Spaulding, P. (1961). Foreign Diseases of Forest Trees of the World: An Annotated List. USDA Agriculture Handbook 197:1–361
[52]. Swofford, D. L. (2002). PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4.0a150. Sinauer, Sunderland, MA. https://doi.org/10.1111/j.0014-3820.2002.tb00191.x
[53]. Takamatsu, S., Niinomi, S., Harada, M., Havrylenko, M. (2010). Molecular phylogenetic analyses reveal a close evolutionary relationship between Podosphaera (Erysiphales: Erysiphaceae) and its rosaceous hosts. Persoonia 24: 38–48. https://doi.org/10.3767/003158510X494596
[54]. Thite, S. V., Kore, B. A., Camacho-Tapia, M., Tovar-Pedraza, J. M. (2017). First report of Podosphaera xanthii causing powdery mildew on Ageratum conyzoides in India. Journal of Plant Pathology 99(2): 533–543. https://doi.org/10.3767/003158510X494596
[55]. Uddin, M., Tareen, J. K., Ahmed, F., Adnan, F., Bazai, M.J., Fareed, S. R., Kakar, H. (2022). Powdery mildew a disease of grapes and the fungicide mode of action: a review. Biosight 03(02): 38–52. https://doi.org/10.46568/bios.v3i2.78
[56]. Walsh, P.S., Metzger, D.A., Higuchi, R. (1991). Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506–513.
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Copyright (c) 2026 Siska Arie Santy Siahaan, Jamjan Meeboon, Iman Hidayat, Kartini Kramadibrata, Ika Rosenta Purba
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